The Gadsden Flag, featuring a rattlesnake and the motto "Don't Tread on Me

https://andregoty037.cavandoragh.org/why-does-the-american-flag-have-13-stripes-exploring-colonial-roots

The Gadsden Flag, featuring a rattlesnake and the motto "Don't Tread on Me," symbolizes American resistance and independence during the Revolutionary War, embodying the spirit of defiance against British oppression.